Osteogenic effect of low-intensity pulsed ultrasound combined with bone morphogenetic protein 2 on human periodontal ligament cells
Liu Jun
Hu Bo
Jiang Xin-yi
Sun Ji-cheng
Deng Feng
Song Jin-lin
Abstract：BACKGROUND:Both of low-intensity pulsed ultrasound (LIPUS) and bone morphogenetic protein-2 (BMP-2) can promote the osteogenic differentiation of human periodontal ligament cels, but there is no report on their combination application. OBJECTIVE:To evaluate the biological effect of LIPUS combined with BMP-2 on osteogenic differentiation of human periodontal ligament cels. METHODS:Human periodontal ligament cels were isolated, cultured, passaged and identified. Passage 4 cels were seeded onto 6-wel plates and divided into four groups: control group, LIPUS group, BMP-2 group, LIPUS+BMP-2 group. Alkaline phosphatase activity was detected by alkaline phosphatase activity measurement kit, and the expression of type I colagen and osteopontin was detected by RT-PCR. RESULTS AND CONCLUSION: After LIPUS, BMP-2, and their combination treatment, the activity of alkaline phosphatase was significantly higher than that in the control group (P < 0.05), and the activity of alkaline phosphatase was highest in the LIPUS+BMP-2 group (P < 0.05). RT-PCR results showed that both of LIPUS and BMP-2 could augment the mRNA expression of type I colagen and osteopontin (P < 0.01), but it was more
<br> distinct when they were used together (P < 0.001). In a conclusion, the combined utilization of LIPUS and BMP-2 shows stronger ability in promoting the osteogenic differentiation of human periodontal ligament cels.
Keywords：SonicationBone Morphogenetic ProteinsPeriodontal Ligament
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1668-1672 )