Changes of cartilage matrix components, related growth factors and inflammatory factors in the temporomandibular joint under high acceleration
Li Hong-shi
Chen Xin
Feng Yan
Yin Yin
Cao Jun-kai
Abstract：BACKGROUND:Under repeated +Gz condition from the strong acceleration, smal traumas resulting from pressures and impacts in various directions are formed inside the temporomandibular joint, thereby leading to temporomandibular joint disorders. OBJECTIVE: To investigate the changes in colagen type II, proteoglycans, colagenase, insulin-like growth factor-1, transforming growth factor-β1, tumor necrosis factor-α, interleukin-2 and interleukin-3 in the cartilage matrix after temporomandibular joint disorder. METHODS:Thirty-six rats were randomly divided into three groups: negative control group, only fixed on the centrifugal machine for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks; +5 Gz group, centrifugation at +5 Gz for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks; +10 Gz group, centrifugation at +10 Gz for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks. Al animals were on an empty stomach for 12 hours prior to daily centrifugation.
<br> Chondrocytes from the temporomandibular tissue were isolated and cultured in vitroto extract total protein that was detected by weste blot assay. RESULTS AND CONCLUSION: Compared with the negative control group, the levels of colagen type II, proteoglycans, colagenase, insulin-like growth factor-1, transforming growth factor-β1 in the cartilage matrix after temporomandibular joint were significantly decreased, but the levels of tumor necrosis factor-α, interleukin-2 and interleukin-3 were significantly increased under repeated +Gz conditions. These findings indicate that under repeated +Gz stresses, a great damage to the cartilage matrix of the temporomandibular joint is found as wel as reduced repair capability and increased inflammatory reactions.
Keywords：Colagen Type IIInsulin-Like Growth Factor ITransforming Growth Factor beta1Tumor Necrosis Factor-alphaInterleukin-2Interleukin-3
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1663-1667 )