Biocompatibility of nano-hydroxyapatite/collagen/silk fibroin composite scaffolds for bone tissue engineering
Nian Zheng-hao
Li Hui
Li Rui-xin
Sun Kai
Li Dong
Xu Cheng
Zhang Xi-zheng
Abstract：BACKGROUND:Composite scaffolds made of two or more materials can meet the requirements of tissue engineering scaffolds based on the complementary properties of various materials. OBJECTIVE: To prepare three-dimensional nano-hydroxyapatite (nHA)/colagen (COL)/silk fibroin (SF) scaffolds and to study their biocompatibility. METHODS: Composite scaffolds were prepared by mixing nHA, COL and SF at different ratios of 1:1:5, 1:2:5, 1:3:5. In order to find out the best mixing ratio of SF, COL and nHA, the porosity, pore size, water absorption and mechanical properties were determined. MC3T3-E1 cels were cultured in the nHA/COL/SF scaffolds composite scaffold (1:2:5)in vitro to observe the biocompatibility. Cel viability was detected after co-culture for 2, 4, 6, 8, 12 days using MTT. RESULTS AND CONCLUSION: It was more suitable for bone scaffolds when the mixed ratio of nHA, COL and SF was 1:2:5. The pore size was 98-260 μm, the porosity was (96.72±2.78)%, and the water absorption was (549.37±35.29)%. The stability of its mechanical properties, compressive strain and elastic modulus and other
<br> indicators were measured by biomechanical testing machine, which are suitable for bone tissue engineering applications. MC3T3-E1 cels grew wel in the nHA/COL/SF composite scaffold, indicating the composite scaffold has good cytocompatibility.
Keywords：Subject headings：ColagenSilkHydroxyapatitesTissue Engineering
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1149-1154 )