Activities of reactive oxygen species and superoxide dismutase in human lung adenocarcinoma cells impacted by moxa smoke solution

Dou Chuan-zi; Wu Huan-gan; Wang Shuo-shuo; Ma Xiao-peng; Huang Yan; Zhao Ji-meng; Hu Zhi-hai; Liu Hui-rong; Cui Yun-hua; Zhou Ci-li; Zhao Chen

doi:10.3969/j.issn.2095-4344.2015.07.014

Abstract：BACKGROUND:The safety study of moxa smoke has been the focus of attention in the moxibustion research in recent years. As an important direction, the effects of moxa smoke on the human organ, tissue and cel s have been discussed. Studies have shown that the solution of moxa smoke can induce apoptosis of human lung adenocarcinoma cel s (A549), but the specific mechanism is stil unclear. <br> OBJECTIVE:To observe the influences of moxa smoke on growth inhibition, intracel ular reactive oxygen species and superoxide dismutase of A549 cel s, and to explore the oxidative damage mechanism of moxa smoke on A549 cel s. <br> METHODS:Moxa smoke was col ected by a portable air sampler, and dissolved in DMEM to different concentrations, as 0.5, 0.4, 0.3, 0.2, 0.1, 0.05, 0.025, 0.01 g/L, which was used to culture A549 cel s. Cel Counting Kit-8 method was employed to test cel growth inhibition rate, 2’,7’-Dichlorofluorescin diacetate method was employed to determinate reactive oxygen species intensity, and spectrophotometric method was employed to measure and calculate enzymatic activity of superoxide dismutase. <br> RESULTS AND CONCLUSION:The results of different concentrations of smoke solution intervening A549 cel s in 4, 6 and 8 hours showed that the concentration of 0.4 g/L and 0.5 g/L on A549 cel s growth inhibition rate was higher than 0.01 g/L and 0.025 g/L (P<0.05). Low concentration of moxa smoke (0.05 g/L and 0.1 g/L) could decrease the intracel ular levels of reactive oxygen species, but there was no statistical y significant difference between them (P>0.05);high concentration of moxa smoke (0.4 g/L) could increase intracel ular levels of reactive oxygen species (P<0.01), and decrease the activity of superoxide dismutase (P<0.01). Moxa smoke is shown to have biological activity on A549 cel s, and the mechanism of high-concentration moxa smoke effect on cel growth inhibition may be attributed to peroxidation.