Reduced glutathione promotes the proliferation and differentiation of osteoblasts under high glucose conditions

Zhang Lin-lin; Zhang Jing-ying; Qu Zhe; Song Ke-dong; Ai Hong-jun

doi:10.3969/j.issn.2095-4344.2015.07.001

Abstract：BACKGROUND:Diabetes can reduce the success rate of implants by affecting osteogenesis. What’s more, it also causes a variety of complications. Reduced glutathione can remove free radicals directly or indirectly by an enzymatic reaction, and thus plays a protective role on osteoblasts. <br> OBJECTIVE:To evaluate the effects of reduced glutathione on the proliferation and differentiation of osteoblasts under high glucose conditions and the influence on bone defects of diabetes rabbits. <br> METHODS:In this experiment, the osteoblasts were divided into three groups:normal control group, high glucose group, high glucose+reduced glutathione group. We observed the proliferation and differentiation of osteoblasts by alkaline phosphatase staining and MTT. The diabetes rabbits which had bone defects were divided into two groups, and the mixture of reduced glutathione and Bioss was used to fil the bone defects in the experimental group to judge the influence of reduced glutathione on osteogenesis based on X-ray observation. <br> RESULTS AND CONCLUSION:Reduced glutathione could effectively promote the proliferation and differentiation of osteoblasts under high glucose conditions. The cel proliferation and differentiation showed more significant increase in the high glucose+reduced glutathione group than the high glucose group (P<0.05). The bone mineral density shadow of the tibial bone defects after treatment with the mixture of reduced glutathione and Bioss was significantly higher than that after treatment with single Bioss (P<0.05). These findings indicate that reduced glutathione can promote the proliferation and differentiation of osteoblasts, and effectively promote the repair of bone defects.