Umbilical cord blood mesenchymal stem cells carrying human telomerase reverse transcriptase for pulmonary arterial hypertension

Xu Ying

doi:10.3969/j.issn.2095-4344

Abstract：BACKGROUND:To protect the pulmonary vascular endothelial cels in the body is an important part to reduce pulmonary circulation pressure and prevent pulmonary hypertension. OBJECTIVE:To observe the therapeutic efficacy of umbilical cord blood mesenchymal stem cel transplantation carrying human telomerase reverse transcriptase (hTERT) in the treatment of pulmonary hypertension in rats. METHODS:Umbilical cord blood mesenchymal stem cels were cultured, purified and transfected with adenovirus carrying hTERT gene. Sixty Sprague-Dawley male rats were randomly divided into model group, blank adenovirus group, adenoviral delivery group, with 20 rats in each group. Then, the rats were respectively injectedvia the jugular vein with 1 mL L-DBEB, 1 mL umbilical cord blood mesenchymal stem cel suspension (1.5×1010/L), and 1 mL hTERT-transfected umbilical cord blood mesenchymal stem cel suspension (1.5×1010/L) after models of pulmonary hypertension were establishedvia intraperitoneal injection of monocrotaline. After 21 days of transplantation, hemodynamic changes, plasma endothelin 1 levels and the hypertrophy index of the right ventricle were detected. RESULTS AND CONCLUSION: There was no difference in arterial blood pressure among the three groups (P < 0.05). In the adenoviral delivery group, the systolic pressure of the pulmonary artery, mean pulmonary artery pressure and plasma endothelin 1 level decreased significantly as compared with the model group, blank adenovirus group (P < 0.05); but no difference was found in the hypertrophy index of the right ventricle (P > 0.05). These findings indicate that umbilical cord blood mesenchymal stem cel transplantation carrying hTERT gene can improve the hemodynamic abnormalities in the body and also protect vascular endothelial cels in the body.