Transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells in the treatment of pulmonary hypertension

Song Yan-feng; Cao Jie

doi:10.3969/j.issn.2095-4344

Abstract：BACKGROUND:Currently, emerging stem cel therapy and gene modification technology have the potential to get a unique effect in the treatment of pulmonary hypertension. OBJECTIVE:To investigate the effects of transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cels on the hemodynamic changes of pulmonary hypertension rats. METHODS:Bone marrow mesenchymal stem cels from male Sprague-Dawley rats aged 3 weeks were cultured and purified in vitro, and then transfected with adenovirus-medicated basic fibroblast growth factor. Sixty Sprague-Dawley rats were enroled to prepare pulmonary hypertension models through intraperitoneal injection of 50 mg/kg monocrotaline and then randomly assigned into three groups: model group treated with transplantation of 1 mL L-DMEMvia the jugular vein, cel transplantation group treated with transplantation of 1 mL untransfected bone marrow mesenchymal stem cel suspension, and transfection group treated with transplantation of 1 mL bone marrow mesenchymal stem cel suspension transfected with basic fibroblast growth factor. RESULTS AND CONCLUSION:After 3 weeks of treatment, there were no differences in the arterial blood <br> pressure of the three groups (P > 0.05); however, the systolic pressure of the pulmonary artery and mean pulmonary arterial pressure were significantly lower in the transfection group than the model group and cel transplantation group (P< 0.05). Hypertrophy index of the right ventricle had no difference among the three groups (P > 0.05). The level of plasma endothelin 1 was significantly lower in the transfection group than the model group and cel transplantation group (P < 0.05). Experimental findings suggest that the transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cels can improve the hemodynamic levels in pulmonary blood vessels of pulmonary hypertension rats to protect vascular endothelial cels and antagonize vascular contraction.