Pure magnesium micro-arc oxidation coating containing nano-SiO2 or nano-TiO2 promotes proliferation and viability of osteoblasts

Liu Ji-guang; Wang Li-feng; Li Mu-qin; Gao Yan; Wang Xiao-wei

doi:10.3969/j.issn.2095-4344

Abstract：BACKGROUND:The micro-arc oxidation technology can enhance the corrosion resistance of magnesium and its aloys to improve the biological properties of the surface. <br> OBJECTIVE: In order to regulate the biological activity of medical pure magnesium, to modify the micro-arc oxidation coating by adding nano-SiO2 and nano-TiO2 into the plating solution, and to study the biological coating effects on osteoblast proliferation and differentiation. <br> METHODS: Round magnesium sheets were divided into three groups, respectively treated with silicate electrolyte containing 7.5 g/L nano-SiO2 (nano-SiO2 group), 4.8 g/L nano-TiO2 (nano-TiO2 group), and nothing (control group). Passage 3 osteoblasts were seeded onto the specimen surface in the three groups to observe the early morphology, proliferation and alkaline phosphatase activity of osteoblasts. <br> RESULTS AND CONCLUSION:Osteoblasts grew wel in the nano-SiO2 group and nano-TiO2 group, presenting with clear outline, and the cels were long spindle- and polygon-shaped. But in the control group, the cels grew poor in the specimen surface. Cel counting kit-8 test showed that the absorbance values and alkaline phosphatase activities were increased with time in the three groups. Compared with the control group, the proliferative activity was higher in the nano-SiO2 group and nano-TiO2 group at days 1, 3, 5 after seeding, while the activity of alkaline phosphatase was also higher in the nano-SiO2 group and nano-TiO2 group at days 3, 5 after seeding. These findings indicate the micro-arc oxidation coatings containing nano-SiO2and nano-TiO2 can promote osteoblast proliferation and osteogenetic activity, which have good biocompatibility.