Autogenous iliac boneversuscollagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite material for repair of unilateral cleft palate in rats

Shen Yue; Ma Hai-ying; Zhang Yan-sheng; Wang Juan; Shi Bing-zheng

doi:10.3969/j.issn.2095-4344

Abstract：BACKGROUND:It has been demonstrated that the effect of colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft is good, but there is no comparative analysis of this composite graft and autologous bone graft. <br> OBJECTIVE:To detect the healing effect of autologous bone graft versus colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft in a rat model of unilateral complete cleft palate. <br> METHODS: Firstly, we established the artificial unilateral complete cleft palate models in 32 Sprague-Dawley rats, and then the established animal models were randomly divided into control group and experimental group. The autologous iliac bone was transplanted into the fissures of control group, and the experimental group received colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft. After that, the activities of serum alkaline phosphatase and tartrate-resistant acid phosphatase, bone mineral densities in the neonatal palate, expressions of osteocalcin, osteoprotegerin, core binding factor, and osteoclast differentiation factor were detected at 1, 2, 3, 4 weeks after treatment. <br> RESULTS AND CONCLUSION: Over time, the alkaline phosphatase activity increased gradualy, while tartrate-resistant acid phosphatase activity decreased. Compared with the control group, the alkaline phosphatase activity was always higher (P < 0.05,P < 0.01) but the tartrate-resistant acid phosphatase activity was lower in the experimental group (P < 0.05,P < 0.01); the bone mineral density increased in both groups, but it was always higher in the experimental group than the control group (P < 0.01). The expression levels of osteocalcin, osteoprotegerin, and core-binding factor gene gradualy rose in both groups, but they were always higher in the experimental group than the control group; in contrast, the expression of osteoclast differentiation factor was decreased in both groups, and it was lower in the experimental group than the control group. These findings indicate that the colagen/hydroxyapatite/recombinant human bone morphogenetic protein-2 composite graft has more advantages compared with bone autograft.