Bio-ceramic combined with bone marrow stromal stem cells and bone morphogenetic protein in repair of large segmental radius defects in rabbits
Wang Tian-sheng
Teng Shou-fa
Wang Fa
Guo Li
Ding Hai-jiao
Liu Peng
Zhang Ying-xia
Cao Yu
Wang Zhan
Abstract：BACKGROUND: Synthetic bio-ceramics as good biological scaffolds can compound with autologous bone marrow stromal stem cels. Bone tissue engineering of their combination for bone graft brings a new hope for large bone defects.
<br> OBJECTIVE:To observe the therapeutic effects and feasibility of bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein in repair of large bone defects in rabbits.
<br> METHODS: Rabbit models of 1.5 cm-long bone defects were made by implanting the middle radial bone in the upper limbs. Left bone defect region served as the experimental group, implanting with bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein. Right bone defect region served as the control group, only implanting with bio-ceramic. At 4, 8, 12 and 24 weeks after model establishment, general observation was performed in both groups. Histological changes were observed with radiographs to compare the repair and healing in the bone defect region.
<br> RESULTS AND CONCLUSION:At 4, 8, 12 and 24 weeks after model establishment, compared with the control group, the repair of bone defects was faster, new bone formation was more, with complete plasticity, broken absorption was more apparent in the experimental group. At 24 weeks, bone defect region was completely bridged with broken ends of fractured bone. Bone was basicaly repaired. Results confirmed that bio-ceramic combined with bone marrow stromal stem cels and bone morphogenetic protein as graft materials in treatment of large bone defects of rabbits had a good therapeutic effect, and the effects were better than bio-ceramic alone.
Keywords：Subject headings:Bone Morphogenetic ProteinsMorphogenesisTissue EngineeringBiocompatible MaterialsOsseointegration
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 347-351 )