The performance research of a mixed material of collagen and clay

Wang Jiu-na; Zhao Xing-xu; Tang Jun-jie; Li Gen; Jiang Tuo-zhi; Zhao Ling; Qin Wen; Zhao Hong-bin

doi:10.3969/j.issn.2095-4344.2014.47.006

Abstract：, 1, 2 (1Colege of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China;2Institute of Bone Injury, Lanzhou General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China) <br> BACKGROUND:Attapulgite clay is light with strong water absorption, and it has viscosity and plasticity in wet condition, which has been widely used in various fields. However, there is rarely report about attapulgite clay as bone repair material. OBJECTIVE:To mix colagen with different proportion of attapulgite clay to fabricate a new type of tissue engineering scaffold material for bone repair. METHODS:Colagen and attapulgite clay were melted by dry weight ratio of 1:0, 5:1, 4:1, 3:1 to fabricate scaffold materials. The apparent characteristics of the materials were observed by scanning electron microscopy, the porosity and water absorption of quality of materials were determined before and after 1% genipin cross-linked. Rat bone marrow mesenchymal stem cels were inoculated on the four different materials. The materials were observed by scanning electron microscopy after 3 days. And the materials were stained by hematoxylin-eosin staining after 7 days. The four different materials cross-linked by 1% genipin were implanted into rats and the degradation of materials was observed 1 month after implantation. RESULTS AND CONCLUSION:Materials were sponge and porous. The porosity of materials was between 60% and 80% before cross-linked, while the porosity of materials was between 40% and 65% after cross-linked. There was no significant difference among four groups (P > 0.05). The water absorption of quality of the materials was significantly reduced after cross-linked (P < 0.05). The colagen-attapulgite clay scaffolds have good biocompatibility and can be completely degraded and absorbed in vivo without inflammation. The colagen-attapulgite clay materials of different ratios have different biodegradation rates.