Effect of platelet-rich fibrin released transforming growth factor beta 1 on the proliferation of bone marrow mesenchymal stem cells in vitro

Chen Cheng; Li Shu-hui; Zhang Wen-li; Li Yi-ming; Zhou Jing; Wu Pei-ling

doi:10.3969/j.issn.2095-4344.2014.45.004

Abstract：BACKGROUND:Bone marrow mesenchymal stem cel s are the ideal cel s for tissue repair. Whether the ability of in vitro proliferation can be enhanced is a key factor to promote tissue repair. <br> OBJECTIVE:To observe the effect of transforming growth factorβ1 on the proliferation of bone marrow mesenchymal stem cel s in vitro. <br> METHODS:Blood samples were taken from the central artery of rabbits to prepare platelet-rich fibrin by centrifugation method which was then placed into fresh DMEM at 37℃for 7, 14, 21, 28 days to col ect exudates. The mass concentrations of transforming growth factor-β1 in the exudates of platelet-rich fibrin were detected. Rabbit bone marrow mesenchymal stem cel s were col ected and cultured in the conditioned medium made by the exudates of platelet-rich fibrin, and the proliferation of cel s was observed. <br> RESULTS AND CONCLUSION:Concentration of transforming growth factorβ1 was increased with time increasing, increased fastest at 21-28 days, and peaked at 28 days. Under the same stimulus concentration, the proliferation of bone marrow mesenchymal stem cel s was reduced at 0-1 day, increased obviously at 1-2 days, and entered into a steady phase at 2-3 days. Under 150 ng/L transforming growth factorβ1, bone marrow mesenchymal stem cel s proliferated fastest. Experimental findings indicate that with the increase of time, the concentration of transforming growth factorβ1 in the exudates of platelet-rich fibrin increase gradual y, and the conditioned media containing different concentrations of transforming growth factorβ1 play different roles in <br> promoting the proliferation of bone marrow mesenchymal stem cel s. Bone marrow mesenchymal stem cel s cultured in the conditioned medium containing 150 ng/L transforming growth factorβ1 for 2-3 days can proliferate fastest.