Nano-hydroxyapatite/polylactic acid biomaterials influence proliferative ability of MC3TC-E1 cells

Li Min; Tang Yuan; Atik Badshah Shaikh; Shi Zhan-jun; Wang Jian

doi:10.3969/j.issn.2095-4344.2014.43.007

Abstract：BACKGROUND:Studies have shown that polylactic acid/hydroxyapatite (PLA/HA) composite materials have structure and properties similar to natural bone, exhibiting bone conductivity and good biocompatibility. <br> OBJECTIVE:To evaluate the biocompatibility of nano-hydroxyapatite/polylactic acid (n-HA/PLA) biomaterials with MC3T3-E1 cells. <br> METHODS:The isolated third generation MC3T3-E1 cells were cultured in complete medium (control group) and n-HA/PLA extract (experimental group) for 3, 5, and 7 days. The material cytotoxicity was determined by cellCounting Kit-8. Expression of alkaline phosphatase, osteocalcin, type I col agen, core binding factorα1/osteoblast-specific transcription factor were tested by Real Time-PCR at days 7 and 14 after culture. MC3T3-E1 cells were inoculated onto the biomaterials of n-HA/PLA. Immunofluorescence and scanning electron microscope were use to observe MC3T3-E1 cellmorphology and attachment at days 7 and 14 after culture. <br> RESULTS AND CONCLUSION:The osteoblast proliferation rates in the experimental and control groups were increased gradual y with time, and there was no difference in the absorbance between the two groups. At day 4, the activity of alkaline phosphatase was higher in the experimental group than the control group (P<0.05);at day 7, the expressions of alkaline phosphatase, type I col agen, core binding factorα1/osteoblast-specific transcription factor were higher in the experimental group than the control group (P<0.05). cells began to grow adherently on the materials at day 7 after co-culture, and present a polygon shape. At day 14, cells on the scaffold increased significantly in number and ful y extended with polygon and fusiform morphology. These results reveal that n-HA/PLA biomaterials have good cellcompatibility and no cytotoxicity.