Dibutyl phthalate influences the secretion of rat ovarian granulose cells
Wang Xue-jin
Zhou Yun-qing
Luo Xiang-min
Liu Jin
Abstract：BACKGROUND:Studies have shown that dibutyl phthalate has certain toxicity to the reproductive system of male animals. <br> OBJECTIVE:To investigate the effects of dibutyl phthalate on secretion of rat ovarian granulose cells. METHODS:Ovarian granulosa cells from 25-day-old female Sprague-Dawley rats were cultured in vitro and exposed to different concentrations of dibutyl phthalate (0, 5, 20, 80μmol/L) for 24 hours. Estradiol and progesterone in ovarian granulosa cells were measured by the method of radio-immunity. Expression of the cytochrome P450aromatase and P450 side chain cleavage enzyme mRNA in granulosa cells were examined by real-time PCR. <br> RESULTS AND CONCLUSION:The levels of estradiol and progesterone in granulosa cells decreased after exposure to dibutyl phthalate. With the increase of the dibutyl phthalate concentration, the expression of <br> P450arom and P450scc mRNA decreased gradual y. Dibutyl phthalate could induce reproductive dysfunction and toxicity to female rats.
Keywords：phthalic acidscytochromesurogenital systemestradiolprogesterone
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 6779-6783 )