Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase
Wu Ling-zhi
Li Shui-bin
Cheng Gang-wei
Wang Hua-qiao
Kong Ling-ping
Abstract：BACKGROUND:Human telomerase reverse transcriptase recombinant adenovirus transfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons.
<br> OBJECTIVE:To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloidβ-protein invention.
<br> METHODS:Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloidβ-protein (25-35) group, and human telomerase reverse transcriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5μmol/L amyloidβ-protein (25-35) for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse transcriptase group at 72 hours after intervention of 5μmol/L amyloidβ-protein (25-35).
<br> RESULTS AND CONCLUSION:After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloidβ-protein (25-35). Human telomerase reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloidβ-protein (25-35).
Keywords：cerebral cortexapoptosistelomerasetelomerase-binding proteinstransfection
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 4653-4657 )