Human adipose-derived stem cells promote angiogenic capability of random pattern flaps in mice

Cao Jing; Jiang Nan; Xu Yang-yang; Zhu Meng-lin; Yang Liu

doi:10.3969/j.issn.2095-4344

Abstract：BACKGROUND:Adipose-derived stem cels are a population of multilineage cels isolated from adipose tissue, which may have a positive effect on the treatment of ischemic diseases. <br> OBJECTIVE:To explore the therapeutic and angiogenic effects of adipose-derived stem celsvia local transplantation on random pattern skin flaps in mice. <br> METHODS: Human adipose-derived stem cels were isolated, cultured and passagedin vitro. On the back of the SPF mice, random pattern skin flaps were performed. After the operation, the adipose-derived stem cels were injected into the pedicle, central, and distal end of the flaps in the experimental group, while only PBS was injected into the control flaps. Seven days later, the survival rate of flaps was evaluated. Immunofluorescence assay was preformed to observe the distribution of microvessels in the flaps and trace the CM-Dil labeled adipose-derived stem cels, while the level of vascular endothelial growth factor was tested by ELISA and the protein expression of stromal cel-derived factor 1 tested by western blot at day 14 after adipose-derived stem cels transplantation. <br> RESULTS AND CONCLUSION:Compared with the control group, there was a significant increase in the flap survival rate in the experimental group, and along with the sharply increased number of microvessels, the secretions of vascular endothelial growth factor and stromal cel-derived factor 1 were also obviously raised in the experimental group (P < 0.05). After local transplantation of adipose-derived stem cels into random skin flaps, it could intervene the secretion of vascular endothelial growth factor and stromal cel-derived factor 1 and promote angiogenesis of the skin flap.