Icariin intervenes wear particles-induced osteolysis

Wang Jun; Zhu Xu-ri; Zhang Chao; Shen Ji-rong; Du Bin

doi:10.3969/j.issn.2095-4344.2014.17.001

Abstract：BACKGROUND:Osteolysis surrounding artificial joints is the main reason for the failure of joint loosening. Scholars al over the world are seeking an effective drug to inhibit osteolysis so as to reduce the occurrence of prosthetic loosening. <br> OBJECTIVE:To observe the effects of different doses of icari n on osteolysis induced by wear particles. <br> METHODS:The wear debris (Ti al oy and bone cement) was put into mouse cranium to create mouse models of calvarial osteolysis. Physiological saline was dropped in the cranium in the blank group. Different concentrations of icari n (30, 60 and 120 mg/kg) were given in the drug intervention group, by intragastric administration, once a day. At 2 weeks after model induction, mice were sacrificed. The structure of mouse cranium was observed under a microscope. Hematoxylin-eosin staining and immunohistochemical staining were performed. The number of osteoclasts and the changes in the calvarial osteolysis area of mice after intervention were calculated. <br> RESULTS AND CONCLUSION:Compared with the blank group, the number of absorption pits of bone slices that were stimulated by wear debris (Ti al oy and bone cement) and the number of osteoclasts were significantly increased, and absorption pits area was also bigger (P<0.05). After intervention with icari n, osteolysis area and the number of osteoclasts became less (P<0.05), especial y in the 120 mg/kg gavage group, fol owed by 60 mg/kg and 30 mg/kg. Results suggested that Ti particles and bone cement particles can promote osteoclast proliferation and osteolysis. Icari n can suppress the formation of osteoclasts induced by particles, resulting in inhibiting osteolysis.