Dexamethasone and Lugua peptide in osteoblast proliferation and differentiation:inhibitive or promotive effects?
Duan Feng
Wen Ke-han
Wang Xin-yu
Zhang Guo-liang
Dai Xin-peng
Guo Huan-huan
Abstract：BACKGROUND:The incidence of osteoporosis caused by long-term use of dexamethasone injection is increasing, but there is no exact treatment. Lugua peptide injection is a common drug to promote fracture healing and increase bone density.
<br> OBJECTIVE:To observe the effect of Lugua peptide injection plus high-concentration dexamethasone on osteoblast proliferation and differentiation.
<br> METHODS:The rat osteoblasts were randomly divided into three groups. Lugua peptide plus dexamethasone group was injected with 1×10-7 mol/L dexamethasone, 8 mg/L Lugua peptide and dimethyl sulfoxide (final concentration 1 g/L). Dexamethasone group was injected with 1×10-7 mol/L dexamethasone and dimethyl sulfoxide (final concentration 1 g/L). Control group was injected with dimethyl sulfoxide (final concentration 1 g/L). After 96 hours, the expression of osteocalcin was analyzed by RT-PCR analysis;at 21 days, the expression of calcium nodules was observed by alizarin red staining.
<br> RESULTS AND CONCLUSION:The osteocalcin mRNA expression and calcium nodule expression in Lugua peptide plus dexamethasone group and control group were higher than that in dexamethasone group (P<0.01), while there was no significant difference between Lugua peptide plus dexamethasone group and control group (P>0.05). Lugua peptide injection can prevent the inhibitory effect of high concentrations of dexamethasone on the osteoblast proliferation and differentiation.
Keywords：dexamethasoneglucocorticoidsosteoblastsosteoporosisosteocalcin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 2314-2319 )