Silane coupler effects on titanium surface modification and biocompatibility

Gulbanu Ymam; Xu Guo-qiang; Dilnur Aji; Guzalay Abdukadr; Liu Jia-yi; Xu Bin; Li Qi

doi:10.3969/j.issn.2095-4344.2014.12.010

Abstract：BACKGROUND:Nowadays, silane coupling for metal surface pretreatment has not been widely applied yet. OBJECTIVE:To observe the surface appearance, structure and biocompatibility of NaOH pre-treated titanium sheet coated with silane films. <br> METHODS:The NaOH pre-treated titanium sheet was silanized with the silane coupler (KH-550) at different concentrations (8%, 15%and 33%). The test specimens manufactured with different concentrations of silane treatment solutions were analyzed with scanning electron microscope and energy dispersive spectroscopy analyzer, in comparison with pure titanium and alkali-pretreated titanium sheets as controls. After silanization, bone marrow mesenchymal stem cel s from dogs were co-cultured with the test specimens to observe the cel morphology and adhesion on the surface of test specimens. <br> RESULTS AND CONCLUSION:The silane film consisted of brain shaped sheets which arranged closely. The composition of the silane film was C, N, O and Si. The test specimen coated with the 33%silane solution had the ful silane film, while the silane film was imperfect at a concentration of 8%. The cel adhesion was better on the surface of test specimens treated with 33%silane solution than the other specimens. These findings indicate that the titanium sheet modified with 33%silane solution possesses a good biocompatibility, thereby improving the cel adhesion, spreading, proliferation and differentiation.