Proteomic difference of serum containing Cinnamon Twig Decoction Plus Pueraria on annulus fibrosus cells

Zhong Wei-hong; Li Yu-tao; Zheng Qi-kai; Ye Jia-jia; Lin Jian-ping; Wang Shi-zhong

doi:10.3969/j.issn.2095-4344.2014.11.013

Abstract：BACKGROUND:Anulus fibrosus cleavage is the main contributing factor of intervertebral disk degeneration in cervical spondylosis. Previous studies of our research group have demonstrated that, Cinnamon Twig Decoction Plus Pueraria is a definitely effective treatment of cervical spondylosis. However, differential proteomic research addressing the intervention of Cinnamon Twig Decoction Plus Pueraria on anulus fibrosus cel s is rarely reported. OBJECTIVE:To explore the target or related proteins of Cinnamon Twig Decoction Plus Pueraria intervention on anulus fibrosus cel s, at the differential proteomics level. <br> METHODS:Anulus fibrosus cel s were col ected from Sprague-Dawley rats that were cultured in vitro. Cultivated anulus fibrosus cel s at passage 3 were randomly divided into two groups:experiment group was cultured in serum containing Cinnamon Twig Decoction Plus Pueraria, while control group in saline. The isotopical y labeled relative and absolute quantification was applied to mark the sample proteins in two groups. Two-dimensional liquid chromatography mass spectrometry was used to identify the proteins and relative quantitative analysis was performed. <br> RESULTS AND CONCLUSION:Compared with the control group, platelet-derived growth factor subunit A, bone morphogenetic protein 2, insulin-like growth factor I, aggrecan core protein, transforming growth factor beta-3, interleukin-1 beta expression were increased in the experiment group, while fibroblast growth factor 2 and col agen alpha-1 chain expression was decreased. Cinnamon Twig Decoction Plus Pueraria can repair the degeneration of anulus fibrosus cel s.