Limb ischemic postconditioning promotes the proliferation of endogenous neural stem cells in rats with cerebral ischemia injury
Du Guo-jia
Dang Mu-ren
Tan Ze-ming
Su Ri-qing
Shen Qian
Fang Jia-sheng
Abstract：BACKGROUND:There are a few reports addressing application of limb ischemic preconditioning in cerebral ischemia-reperfusion injury, but few reports have addressed limb ischemic postconditioning applied in this field. OBJECTIVE:To explore the effects of limb ischemic postconditioning on the proliferation of neural stem cells of rats after cerebral ischemia. METHODS:Focal stroke models were established in rats, and then 30 rat models were randomly divided into experimental group (n=15) and control group (n=15). Remote limb ischemic postconditioning was applied in the experimental group, but not in the control group. 5-Bromo-2-deoxyuridine (BrdU;50 mg/kg) was injected intraperitoneal y into rat models three times a day, and then the rat brains were respectively obtained at days 5, 10 and 15 days in two groups. The amount of BrdU-positive cells was measured by immunohistochemistry method in two groups.
<br> RESULTS AND CONCLUSION:Compared with the control group, the experimental group was significantly decreased in the severity of injury and behavior scores (P<0.05). At various time points, the number of BrdU-positive cells was obviously greater in the experimental group than the control group (P<0.05). Results have indicated that limb ischemic postconditioning can improve behavior of rats and promote the proliferation of neural stem cells, thereby showing neuroprotective effects against cerebral ischemia injury.
Keywords：stem cellsneural stem cellsischemic postconditioningbrain injuriescell proliferationbromodeoxyuridine
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 1597-1602 )