Biocompatibility of hydroxyapatite/TiO2 nanotube composites

Zhang Hang-zhou; Sun Yu; Wang Lin; Tian Ang; Xue Xiang-xin; Bai Xi-zhuang

doi:10.3969/j.issn.2095-4344.2014.03.002

Abstract：BACKGROUND:Hydroxyapatite has excellent biocompatibility, but biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites is rarely reported. <br> OBJECTIVE:To evaluate the biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites. <br> METHODS:First, the TiO2 nanotubes were fabricated on the surface of the titanium by anodic oxidation <br> technique. Second, the nano-hydroxyapatite/TiO2 nanotube composites were fabricated by electrodeposition technique. The surface morphology of the composites was observed by scanning electron microscopy. Mouse osteoblasts MC-3T3-E1 were co-cultured with the nano-hydroxyapatite/TiO2 nanotube composites, TiO2 nanotubes and titanium, and commercial y pure titanium to observe the celladhesion, proliferation and necrosis on scaffolds. <br> RESULTS AND CONCLUSION:The morphology of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites could be control ed by altering the conditions of the anodic oxidation and electrodeposition. Under the inverted microscope, after 3 days of co-culture with TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites, MC-3T3-E1 cells proliferated wel with regular shape and arrangement that were superior to those on commercial y pure titanium. Under scanning electron microscope, the cellwere adhered and proliferated wel on the surface of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites after 3 days. Apoptosis rate of the cells was significantly reduced on the surface of nano-hydroxyapatite/TiO2 nanotube composites (7.8%) compared with TiO2 nanotubes (9.4%) and commercial y pure titanium (13.5%), indicating nano-hydroxyapatite/TiO2 nanotube composites have good biocompatibility.