Time for establishing an animal model of acute high intraocular pressure

Zhang Kun-ya; Qian Xiu-qing; Liu Zhi-cheng

doi:10.3969/j.issn.2095-4344.2013.46.011

Abstract：BACKGROUND: An animal model of acute high intraocular pressure offers an easy and effective method to glaucoma research. Research results will be distorted if the animal model is not induced successfully. Nowadays, a successful animal model can only be identified by observational indexes. <br> OBJECTIVE: To observe the induction time for an animal model of acute high intraocular pressure, and thereby to provide accurate and feasible method for identifying whether the animal model is induced successfully. <br> METHODS: Acute high intraocular pressure animal models were induced by anterior chamber perfusion using communicating vessels-based device with a glass vessel filled with normal saline and 2-mm-long mercury. Liquid volume was perfused to rabbit eyes. A total of 10 animal models were prepared successfully at an intraocular pressure of 4.9 kPa. The experiment was complete till 30 minutes after the perfused liquid was not moved. At the certain intraocular pressure, the variation of fluid inflow over time was recorded, and the induction time for successful modeling was calculated. <br> RESULTS AND CONCLUSION: The curve between fluid inflow into rabbit eyes (y) and induction time (t) could be fitted to logistic function, while U, b0 and b1 are 957.44±313.22, 0.023±0.008 and 0.980±0.003, respectively. The fluid inflow to the eye ball did not change when the induction time was over (282.93±42.68) minutes, and the animal model of acute high intraocular pressure was established successfully.