Dexamethasone induces osteogenic differentiation and apoptosis of periodontal ligament stem cells
Gu Zi-ya
Zhai Qiang
L Qiu-feng
Peng Can-xing
Liu Fang-fei
Peng Hui
Abstract：BACKGROUND:Periodontal ligment stem cells are adult stem cells derived from dental tissues, which possess the capability of osteogenic differentiation. They are promising“seed cells”in bone tissue engineering. <br> OBJECTIVE:To investigate the effects of osteogenic induction medium on the osteogenic differentiation and early apoptosis of periodontal ligment stem cells. <br> METHODS:Periodontal ligment stem cells were isolated from primary periodontal ligament tissues and seeded on the plate at a density of 1×104/cm2. Osteogenic induction medium containing 1, 10 and 100 nmol/L dexamethasone,β-glycerophosphate and ascorbic acid were used. Alkaline phosphatase activity, mineral deposition staining and real time PCR were performed to assess the osteogenic capacity. AnnexinV/PI staining was used to detected apoptosis. <br> RESULTS AND CONCLUSION:Dexamethasone effectively promoted osteogenic differentiation of periodontal ligment stem cells, by up-regulating alkaline phosphatase activity, enhancing mineral deposition and increasing osteogenic related gene (osteonectin and type Ⅰ col agen) expression. Alkaline phosphatase activity and mineralized nodule detection showed that osteogenic effects of dexamethasone were gradient-dependent, and 100 nmol/L was the best concentration. Apoptosis detection results indicated that osteogenic differentiation of dexamethasone for periodontal ligment stem cells could also induce cellapoptosis to some extent.
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Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 7090-7095 )