Expression of serum and glucocorticoid-regulated protein kinase 3 in breast cancer stem cells

Xu Hai

doi:10.3969/j.issn.2095-4344.2013.40.007

Abstract：BACKGROUND:Serum and glucocorticoid-regulated protein kinase 3 may be related to tumor progression, and can prevent the apoptosis of breast cancer cells induced by interleukin-3 withdrawal. Serum and glucocorticoid regulated protein kinase 3 possibly participate in the regulation of intracellular Wnt signal pathway. <br> OBJECTIVE:To investigate the expression of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin protein expression in breast cancer cells. <br> METHODS:Breast cancer stem cells were isolated, identified and subcultured from 71 breast cancer cases by dual-wave flow cytometry. The 30 normal breast tissues were used as the controls. SP immunohistochemistry analysis was used to detect the level of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin protein expression in breast cancer stem cells and normal breast tissues. <br> RESULTS AND CONCLUSION:The expression of serum and glucocorticoid-regulated protein kinase 3 andβ-catenin in breast cancer stem cells were both higher than that of normal breast tissues (P<0.01). There had a positive correlation between serum and glucocorticoid-regulated protein kinase 3 andβ-catenin expression in breast cancer stem cells (r=0.318, P<0.05). Serum and glucocorticoid-regulated protein kinase 3 may play an important role in the occurrence and development of breast cancer by regulation of Wnt/β-catenin pathway.