Degradation of carboxymethyl chitosan by several common periodontal pathogens
Cao Wen-shuai
Deng Jing
Xu Quan-chen
Zhu Yuan-qi
Abstract： BACKGROUND:In recent years, the degradation in vivo of carboxymethyl chitosan has become a research focus in the fields of periodontal tissue engineering and materials science. However, in the literature involving carboxymethyl chitosan enzymatic degradation, there is no report regarding whether common periodontal
<br> pathogens could degradate carboxymethyl chitosan.
<br> OBJECTIVE:To study the degradation of carboxymethyl chitosan by several common periodontal pathogens. METHODS:1%and 2%carboxymethyl chitosan anaerobic microbiological culture media were prepared. The colonies of Porphyromonas gingivalis, Actinobacil us actinomycetemcomitan and Actinomyces viscosus were picked up streaking onto the carboxymethyl chitosan medium, respectively, which was then incubated in
<br> anaerobic culture bag at 37 ℃. If the see-through circle appeared around the colonies, the result was positive, indicating that the bacteria can degradate carboxymethyl chitosan;on the contrary, it was negative. Afterwards, the diameter of the colonies was measured.
<br> RESULTS AND CONCLUSION:It was observed that the colonies of Porphyromonas gingivalis grew wel in the 1%carboxymethyl chitosan medium and the see-through circles appeared around the colonies after incubation for 18 days;meanwhile, the medium containing 2%carboxymethyl chitosan inhibited the growth of
<br> Porphyromonas gingivalis and the standard morphological colonies did not form wel . The see-through circle did not appear around the colonies of Actinobacil us actinomycetemcomitan and Actinomyces viscosus in the two
<br> concentrations of carboxymethyl chitosan media and the colonies were dried-up and lost activity after 2-week incubation. The results indicated that the lower concentration of carboxymethyl chitosan could induce Porphyromonas gingivalis to produce chitosanase and degradate carboxymethyl chitosan. Neither Actinobacil us actinomycetemcomitan nor
<br> Actinomyces viscosus could degradate carboxymethyl chitosan.
Keywords：biomaterialsbiomaterials and drug controlled releasemembrane biomaterialscarboxymethyl chitosanperiodontal pathogensPorphyromonas gingivalischitosanasesee-through circlecolonies
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 6152-6158 )