Adipose-derived stem cells combined with gelatin sponge for repair of rabbit radial defects
Liu Gang
Wang Ming-xing
Yan Chang-ming
Wang Yun-qing
Wei Xue-jie
Hu Zhao-li
Abstract： BACKGROUND:Adipose-derived stem cells discovered recently are a new kind of adult stem cells, and have a strong multi-differentiation capacity. However, there are rare studies concerning in vivo osteogenic capacity of adipose-derived stem cells. <br> OBJECTIVE:To investigate the effect of adipose-derived stem cells combined with gelatin sponge on repairing bone defects. <br> METHODS:Adipose-derived stem cells from rabbit inguinal fat pads were isolated and cultured, and then <br> induced using an osteogenic medium containing bone morphogenetic protein 2 fol owed by injection of gelatin sponge. Radial defect models of rabbits were prepared. Compound of adipose-derived stem cel s and gelatin sponge was implanted into the lesion side, while gelatin sponge alone was implanted into the contralateral side. Rabbits were kil ed at weeks 6 and 12 after bone defect repair for X-ray examination, CT scan, and hematoxylin-eosin staining. <br> RESULTS AND CONCLUSION:Lane-Sandhu X-ray and Lane-Sandhu histological scores after compound implantation were significantly higher than those after repair with gelatin sponge alone. It indicates that <br> adipose-derived stem cel s combined with gelatin sponge can promote bone defect healing of rabbits, showing an obvious osteogenic capacity in vivo.
Keywords：biomaterialstissue-engineered bone materialsadipose-derived stem cellsradial defectsbone morphogenetic proteincell culturehistologyradiology
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 6083-6088 )