Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material
Liu Xian-ning
Zhu Xiu-ping
Wu Jie
Wang Li-fang
Yin Yong
Abstract： BACKGROUND:Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material. <br> OBJECTIVE:To determine the potential toxic effects of ostrich corneal stromal scaffold on cel s. <br> METHODS:Cel culture methods were used to culture L-929 cel s in the extracts of ostrich acel ular corneal <br> stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cel s after cultured for 1, 2 and 3 days. <br> RESULTS AND CONCLUSION:After the cel s were cultured in the extracts of ostrich acel ular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cel s was graded as level 1. The cytotoxicity test was conducted according to the“National Standard of the People's Republic of China GB/T16886.5-2003”. After cultured in the extracts of ostrich acel ular corneal stroma, a smal number of cel s were round in shape and loosely adherent without intracytoplasmic granules, and cel lysis could be observed <br> occasional y. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that <br> the ostrich acel ular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.
Keywords：tissue constructioncytology experiment in tissue constructiontissue-engineered corneacorneaostrichacel ular corneal stromascaffolddehydrationpreservationcytotoxicity testprovincial grants-supported paper
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5995-6000 )