Streptococcus mutans gcp gene knockout strains expression profile gene chip
Xie Miao-miao
Hu Xiao-cong
Wu Bu-ling
Yan Wen-juan
Abstract： BACKGROUND:Previous studies have confirmed the presence of bis-(3'-5')-cyclic dimeric guanosine <br> monophosphate signaling pathway in Streptococcus mutans, which construct the streptococcus mutans gcp gene knockout strains. <br> OBJECTIVE:To compare the gene expression differences between Streptococcus mutans wild strains and gcp mutant strains, and to screen the biofilm-related genes from them for the fol ow-up study. <br> METHODS:The total RNA of two kinds of strains were extracted and stained with cy3 and cy5 respectively after reverse transcription. The gene chip was scanned after hybridization and the differential gene were obtained <br> through the data analysis. The different expression genes were verified by real-time PCR. <br> RESULTS AND CONCLUSION:Differential genes were mainly relative about glucose metabolism and biofilm formation. We selected two genes for real-time PCR verification. The PCR results were consistent with the <br> microarray results. After Streptococcus mutans gcp gene knockout, the gene expressions of gcp mutant strains were upregulated and the gene expressions of phosphotransferase system were downregulated, this result <br> suggested that two different genes were related with the c-di-GMP signal pathway downstream.
Keywords：tissue constructioncytology experiment in tissue constructionStreptococcus mutansgene chipc-di-GMP signal pathwayahpCphosphotransferase systemNational Natural Science Foundation of China
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 5988-5994 )