Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts
Huang Yun-mei
Chen Wen-lie
Lin Ru-hui
Huang Mei-ya
Li Zuan-fang
Liao Nai-shun
Liu Xian-xiang
Abstract： BACKGROUND:Tougu Xiaotong capsule is the clinical prescription for the treatment of osteoarthritis in Fujian University of Traditional Chinese Medicine, and the previous studies mainly focus on effect to cartilage. <br> OBJECTIVE:To observe the effect of Tougu Xiaotong capsule on the proliferation and differentiation of osteoblasts as wel as the expressions of bone remodeling correlated factors. <br> METHODS:Rat osteoblast-like cel line ROS17/2.8 cel s were incubated with Tougu Xiaotong capsule. The ROS17/2.8 cel s were divided into blank control group and Tougu Xiaotong capsule groups with different <br> concentrations. The cel proliferation was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. Osteoblast differentiation biomarkers alkaline phosphatase activity, osteocalcin and bone mineralized nodules were measured with colorimetry, enzyme-linked immunosorbent assay and alizarin red staining, respectively. The real-time PCR and enzyme-linked immunosorbent assay were used to detect the expressions of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand. <br> RESULTS AND CONCLUSION:Compared with the control group, the Tougu Xiaotong capsule with the <br> concentration of 0.25-2 g/L could significantly promote the ROS17/2.8 cel proliferation (P<0.05), up-regulate the alkaline phosphatase activity, osteocalcin expression level and mineralized nodules area, and increase the <br> percentage of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand (P<0.05). The mechanism of Tougu Xiaotong capsule protecting osteoarthritis may partly result from the regulation of <br> proliferation and differentiation of osteoblasts and bone remodeling.
Keywords：tissue constructionbone tissue constructionTougu Xiaotong capsuleosteoarthritissubchondral boneosteoblastsproliferationosteoprotegerin/receptor activator of nuclear factorκB ligandbone remodelingprovincial grants-supported paper
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 5923-5928 )