Insulin-like growth factor 1 affects the apoptosis of rat condylar chondrocytes
Wei Li
Jiang Li-ting
Zhou Qi
Zhu Ya-ping
Gao Yi-ming
Abstract： BACKGROUND:Insulin-like growth factor 1 is the key factor during cartilage development, which is involved in the growth and reconstruction of condylar cartilage. <br> OBJECTIVE:To study the effect of insulin-like growth factor 1 on cel apoptosis and the apopotosis-associated factors of Bcl-2, Bax mRNA and protein expressions of rat condylar chondrocytes. <br> METHODS:The 1-day-old and 28-day-old rat condylar chondrocytes were cultured and identified in vitro. The condylar chondrocytes with different ages were divided into experimental group and control group. After being starved for 24 hours, chondrocytes in the experimental group were incubated with 100μg/L recombined rat insulin-like growth factor 1 for 48 hours, while the chondrocytes in the control group were incubated normal y. RESULTS AND CONCLUSION:Compared with the control group, after being incubated with recombined <br> insulin-like growth factor 1, the number of condylar chondrocytes was increased with high speed proliferation (P<0.05). Real-time RCR and western blot analysis revealed that the expression levels of Bcl-2 mRNA and protein were increased after added with recombined rat insulin-like growth factor 1, while the expression levels of Bax and protein were decreased (P<0.05). The results indicate that insulin-like growth factor 1 can promote the <br> proliferation and reduce cel apoptosis of newborn and adolescent rat condylar chondrocytes, which may be mediated by Bcl-2 and Bax.
Keywords：tissue constructioncartilage tissue constructioninsulin-like growth factor 1mandibular condylechondrocytescel apoptosiscel proliferationBaxBcl-2provincial grants-supported paper
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 5901-5908 )