Effect of He-Ne laser radiation on the monoamine in mouse brain

doi:10.3321/j.issn:1673-8225.2004.04.019

Abstract：BACKGROUND: It is known that monoamine may take part in the regulation of various functional activities of the body and also in the pathophysiological processes, but the reports about the influnences of low-power laser radiation over monoamine are still rare. Moreover, the results gained are very inconsistent, and some are even contradictory to each other.OBJECTIVE: To elucidate whether low power He-Ne laser radiation can affects serotonin(5-HT) and dopamine(DA) in brain.DESIGN: A completely randomlized study based on the experimental.SETTING AND MATERIALS: The experiment was done in the Central Laboratory of the First Hospital of Beijing University. Materials used were 90Kunming male mice with body-weight 20 -27 g, supplied by Beijing University Animal Centre.INTERVENTIONS: The mice were randomly assigned to 5 groups with randomized arithmetic form. They are normal control group, sham radiation group, aser irradiation group A(13.8 J/cm2), aser irradiation group B (27.6 J/cm2) and laser irradiation group C( 55.1 J/cm2) . The cranial bone and brain of the mouse was exposed under mild anesthesia and irradiated on the sinciput with different doses of He-Ne laser. Then the mice were killed by decapitation. Living creature samples were made.MIAN OUTCOME MEASURES: The cerebral serotonin and dopamine were observed and measured by means of high performance liguid chromatography-electrochemistry ( HPLC-EC ).RESULTS: Dopamine contents(ng/g) were 485.4 + 10. 1 in laser group A,505.8 + 10. 3 in laser group B and 524. 1 ± 10.5 in laser group C, with significant difference as compared with that of the sham laser group(446.5 +7.1)(P ＜0.05, F=4.64; P ＜0.01, F=8.63;P ＜0.01, F=14.52, respectively) . Serotonin contents(ng/g) were 1 283.8 +25.1 in laser group A, 1 337.0 ± 26. 8 in laser group B and 1 429.9 ± 30. 1 in laser group C, significantly higher than that of the sham laser group(1 198.0 ±28.5 )(P ＜0.05, F=4.92; P ＜0.01, F=9.75; P ＜0.01, F=16. 83, respectively). It was found that there was a significant increase in the cerebral serotonin and dopamine in the irradiated mice, and within the laser dose range used, the larger is the laser dose was, the more DA and 5-HT are.CONCLUSION: He-Ne laser radiation may cause a significant increase in 5-HT and DA in the brain of the mice, and the increase is related to the dose of laser radiation.