Effects of interleulin-6 on neurocyte FOS expressions after peripheral nerve injury

doi:10.3321/j.issn:1673-8225.2003.32.008

Abstract：BACKGROUND:Low- level FOS expressions of neural system under normal circumstance increase after injury.Interleulin- 6(IL- 6)can inhibit neurocyte FOS expressions after the injury of central neural system;however,there is no report on the relationship between IL- 6 and neurocyte FOS expressions after peripheral nerve injury. OBJECTIVE:To observe the expression of neurocyte FOS as well as the effect of IL- 6 on neurocyte FOS expression after peripheral nerve injury. DESIGN:Forty- eight SD rats were randomly divided into two group. SETTINGS AND PARTICIPANTS:The research was completed in Guangdong Medical College experimental animal center from August to December 2002.Objects:48 SD rat with a weight between 250 g and 340 g, either male or female were selected for the research. INTERVENTIONS:The left sciatic nerve of rat was exposed under asepsis condition;2 ml IL- 6 was injected into the nerve of intervention group as well as 2 ml saline was injected into the same site of control group. The sciatic nerve was injured by extrusion 1 hour after the surgery, and the nerve was taken for immunohistochemical staining at 1,2,3,4 hours respectively after the injury. MAIN OUTCOME MEASURE: The FOS immune reaction(FOS- IR) positive cells were observed in both intervention and control group. RESULTS:The FOS- IR positive cells in control group at 1,2,3,4 hours after injury were(548± 14, 640± 12),(718± 21),(732± 11)as well as that of the intervention group were(202± 13), (212± 17),(245± 22)and(288± 14) respectively,which the values were increased along with the prolongation of the extrusion time.The FOS- IR positive cells in intervention group at different time were significantly less than those of the control group(t=- 1.812, 2.128,P< 0.05;t=- 2.864, 3.159,P< 0.01). CONCLUSIONS:IL- 6 has an inhibitory action on neurocyte FOS expressions after peripheral nerve injury, which suggests that IL- 6 participates in the control process after nerve injury.