Influence of Naoerkang on the intracerebral cholinesterase activity and neurons of mice with Alzheimer's disease

doi:10.3321/j.issn:1673-8225.2003.31.007

Abstract：AIM:To study the effect of Naoerkang in protecting mice against learning and memory disorders and its influence on the cholinesterase activity and its mechanism of antidementia. METHODS:Sixty healthy male mice of Kunming species were randomized into 6 groups.Except the blank control group,after making model continuously for 50 d,3 mice were selected randomly from each group,with hematoxylin eosin staining,for observing the quantity and appearance of neurocytes in the hippocampal CA1 region.The heads of other rats were cut immediately with their brains taken out,separating the hippocampus and cortexes for testing the acetylcholinesterase activity. RESULTS:The acetylcholinesterase activity of model group was greatly strengthened[cerebral cortex and hippocampous:(3.0± 0.1) and(2.7± 0.1)mol/s in the model group;(2.2± 0.1)and (2.2± 0.1)mol/s in the blank group],(q=16.7,48.2,P< 0.01).To compare the medicinal groups with the model group,except the small dosage group,the acetylcholinesterase activity was much lower than that of the model group(q=7.9- 37.9,P< 0.01);the number of neurons in the hippocampal CA1 area of the model group[(47± 4)/200 μ m]was obviously less than that of the blank group[(101± 11)/200 μ m](q=5.39,P< 0.01),and the neuron loss and the gliocyte proliferation existed.Comparing the medicinal groups with the model group,only in the big and medium dosage groups,the number of neurons was obviously more than that of the model group(q=3.4,P< 0.05 or q=4.3,P< 0.01). CONCLUSION:Naoerkang is able to decrease the acetylcholinesterase activity in the cerebral cortex and hippocampus of AD model mice and protect the neurons in the hippocampal CA1 region.