Protective effect of tetramethylpyrazine on apoptosis of vascular endothelial cells induced by arachidonic acid
Abstract：AIM:To study the protective effects and mechanism of Ligustrazine against damage and apoptosis in human umbilical vascular endothelial cells(HUVECs) induced by 160 μ mol/L arachidonic acid(AA). METHODS:The survival rate of cells was detected by MTT,the release rate of lactate dehydrogenase(LDH) and malondialdehyde(MDA)content in endothelial cells(EC) are measured by colorimetric assay.Hoechst 33258 fluorescence staining was used to observe morphological changes of nucleus and determine apoptotic ratio.Agarose gel electrophoresis was used to detect DNA fragmentation. RESULTS:MP(0.25- 1.0 mmol/L) prevented the inhibitory effect of viability induced by AA(t=2.52- 3.14,P< 0.05).TMP(0.25- 1.0 mmol/L) partly prevented the increase in the release rate of LDH and MDA contents induced by AA(t=3.27- 5.88,t=4.64- 7.41,P< 0.05).Cells treated with 160 μ mol/L AA showed typical morphological changes of apoptosis.A " DNA ladder" was also observed.TMP(0.25- 1.0 mmol/L) partly decreased the ratio of apoptotic cells. CONCLUSION:TMP can protect AA induced apoptosis in EC and the mechanism of its action may be related to anti lipid peroxidation.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 4066-4067 )