Changes of intracellular Ca2+ in the neurons following brain trauma and the contributing factors
Abstract：AIM:To observe the changes of free calcium in rat brain neurons cultured in vitro following blast injury induced by fluid percussion and to explore the protective effect of mild hypothermia, nimodipine (Nim) and D-2-amino-5-phosphonovaleric acid(D-AP-5) on [Ca2+ ]i after injury. METHODS:Changes of [Ca2+ ]i in the rat brain neurons following fluid percussion injury was measured by means of a confocal laser scanning microscope using Fluo-3/AM as a calcium fluorescent indicator. RESULTS:[Ca2+〗 i in the injured neurons increased acutely,reaching the peak level[(411.29± 52.46 nmol/L)] at 24 h after injury,and decreased gradually afterwards,but still maintained higher levels [396.53± 51.32) nmol/L] at 48 h after the injury.Nim and D-AP-5 decreased [Ca2+ ]i in the injured neurons at each time point following the injury.Application of Nim was recommended as early as possible following the injury,and relative poor effect may be expected within 10 h after injury[Ca2+ at (98.65± 15.65) nmol/L 6 h after the injury].Optimal occasion for application of D-AP-5 was between 1 and 10 h after the injury.Hypothermia decreased [Ca2+ ]i markedly within 30 min post injury(t=13.74, P< 0.001)but seemed useless till 1 h following the injury, and its optimal application was within 15 min after the injury. CONCLUSION:The therapy for neuronal calcium overload following TBI must combine multiple therapeutic approaches with their respective optimal time windows observed.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 3810-3811 )