Significance of vector construction of bcl-2 transgene expression for protection against ischemia-reprefusion brain injury
Abstract：AIM:To construct transgenome expressional vector of human bcl-2 gene applied to construct transgenic animals. METHODS: The cDNA primer which include bcl-2 gene was amplified in the blood of chronic lymphatic leukemia patients with RT-PCR. A DNA fragment about 708 bp was acquired from it, and its sequences and isogenies were measured and analyzed after cloning. RESULTS:The production of gene amplification with RT-PCR was identical to the expectation in size. The sequence isogenies of object gene with that of reference gene were 99% . CONCLUSION:Using pEGFP-C1 vector as eukaryotic expressional vector and forming fused gene with green fluorescent protein can be used in the studying of transgenome.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 3592-3593 )