Promoting effect of acupuncture on learning memory of rats and its anti oxidation effect
Abstract：AIM:To investigate the effect of acupuncture on the learning and memory of rats and analyze the relationship between the effect and reaction of free radicals. METHODS:Forty healthy rats with similar body mass and flexibility were randomly divided by number table into 4 groups with 10 rats in each group.In acupuncture group,cosmetological needles were punctured into Shenshu and Paihui of rats which were fixed on self made fixator.Needles were punctured forward and horizontally into Paihui for 3- 5 mm while inward and obliquely into Shenshu for 5 mm.G6805 electric stimulator was connected with continuous wave,frequency of 3 Hz.With shaking of limbs as intensity standard,rats were stimulated once a day for 7 days;Nimodipine group,nimodipine suspension,10 mL/kg,was injected once a day for 7 days.Animals in model group and blank group were fixed with same method from 1 day,10 min each time,once a day and for 7 days.Water maze was used to test the learning memory of normal rats and dementia rats induced by sodium nitrite. The content of brain Lipoperoxide(LPO) and the activity of Superoxide dismutase(SOD) of the rats were also determined. RESULTS:At 5 day,rats in acupuncture could land at (9.3±2.6) s while in blank group,it was (45.5±7.6) s,suggesting that acupuncture could improve the learning memory of normal rats.The landing time was kept in a longer time in model group while the landing time was gradually shortened in acupuncture group(t=2.982, P< 0.05).Besides,in 12d test,the mistake times was (5.71±1.37) while in acupuncture group,it was (0.73±0.25). The difference was significant(t=3.576,P<0.01),suggesting that acupuncture can antagonize the destruction of space learning and memory consolidation by soldium nitrite. In the acupuncture group, The content of LPO [(15.4±0.4) μ mol/g] in the brain cortex was lower than that [(17.2±0.6) μ mol/g] in the model group, but the SOD activity[(101.9±2.2) pmol/(s@g)] was higher than that[(70.4±1.8) pmol/(s@ g)] in the model group (P< 0.05 or P< 0.01).SOD/LPO was negatively correlated to learning ability. CONCLUSION:There is some relationship between the effect of acupuncture in promoting the learning of rats and its effect of inhibiting brain free radical reaction.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3450-3452 )