Effects of 17β- estradiol on the gene expression of bone morphogenetic protein receptor
Abstract：AIM:To investigate the effects of 17β- estradiol (E2) on the gene expression of typeⅠ A and typeⅠ B bone morphogenetic protein receptor (BMPR-Ⅰ A,Ⅰ B) in rat bone marrow stromal cells exposured to the differentiation medium and to elucidate the effects of E2 on osteoblastogenesis. METHODS:Adherent bone marrow stromal cells were cultured in differentiation medium containing DEX(1× 10- 7 mol/L)and 1,25(OH)2D3 (1× 10- 9 mol/L )and different concentrations of E2.The gene expression of BMPR-Ⅰ A,Ⅰ B was quantified by semiquantitative RT- PCR and demonstrated by Northern blot. RESULTS:E2 evidently inhibited the expression of BMPR-Ⅰ A mRNA in bone marrow stromal cell.The suppression was dose dependent.When examined under various concentrations of E2 (0- 1× 10- 6 mol/L),the expression of BMPR-Ⅰ A mRNA were decreased from (27.0± 3.4)% to (22.3± 2.6)% (t=2.2,P >0.05),(17.0± 1.8)%( t=5.2,P< 0.01) and (9.3± 1.6)%( t=9.2,P< 0.01) .Increase of BMPR-Ⅰ B mRNA with increasing of E2 concentration[from (2.0± 0.8)% to (4.8± 1.5)% ,t=3.3,P< 0.05],(8.4± 1.4)%( t=7.9,P< 0.01) and (17.3± 2.2)%( t=13.1,P< 0.01) above the control. Northern blot showed the expression of BMPR-Ⅰ A mRNA decreased from 4.21± 0.36 to 1.24± 0.10( t=18.2,P< 0.01) and the expression of BMPR-Ⅰ B mRNA increased from 1.72 ± 0.11 to 3.73± 0.31( t=12.2,P< 0.01) .The activity of ALP decreased significantly at higher concentrations of E2,from (42.6± 2.5) U/g to (17.9± 2.0) U/g(t=15,P< 0.01), (10.8 ± 1.5) U/g(t=22,P< 0.01) and (3.6± 0.7) U/g( t=30,P< 0.01) . The amount of type Ⅰ collagen was suppressed by E2. CONCLUSION:E2 can suppress the gene expression of BMPR-Ⅰ A of bone marrow stromal cells and inhibited osteoblastogenesis in vitro.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3302-3304 )