Effects of fibronectin on the induction of neural precursor cells from mouse embryonic stem cells
Abstract：AIM:To observe the effect of ITSF and N2 serum-free medium on the differentiation of neural precursor cells from mouse embryonic stem cells and the influence of fibronectin. METHODS:Culture of mouse embryonic stem cell and its differentiation to neural precursor cells with serum-free medium. The embryonic stem cell was stained with NBT/BCIP and the neural precursor cells were stained with immuocytochemistry of nestin. RESULTS:It was found that culturing of embryonic bodies with the bacterial dishes or with hang-in drop method, ITSF or N2 serum-free medium induced mouse embryonic stem cell to neural precursor cells. The number of survive cells in serum-free culturing increased after the surface of dishes was treated with fibronectin, however, the number of embryonic bodies pasted wall had no changes. CONCLUSION:Both ITSF and N2 medium supporte the selectively growth of neural precursor cells, supplement of fibronectin in N2 medium has better effects.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3170-3171 )