Preliminary observation of the effect of transplantation of human embryonic OECs on adult SD rats' semitransected spinal cord
Abstract：AIM:To observe the effect of transplantation of human embryonic OECs on adult rats' semitransected spinal cord. METHODS: OECs,separated from human embryonic olfactory bulb and purified by culture in vivo for 2 weeks,were transplanted into SD rats'(n=10) semitransected spinal cord at T10 level,while the vehicle solution (DMEM) was injected in the controls(n=10).Samples were procured at 6 week after transplantation and studied by nerve silver staining and myelin basic protein(MBP) histochemical staining and nerve growth factor receptor(NGFR) immunohistochemical staining. RESULTS:The injured spinal cords displayed initial healing in OECs transplanted rats 6 weeks post-transplantation morphologically.Histological observation showed that the transplanted OECs integrated into the host tissues in well arrangement and the regenerative axons grew into the distal end of the semitransected spinal cords.Both of OECs and regenerative axons positively expressed MBP and NGFR.In control group, the ends of the impaired spinal cords exhibited liquefied necrosis and axonal degeneration and no regnnerative axons were found. CONCLUSION:OECs transplantation could protect the impaired spinal cord and promote the regeneration of axons.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 3174-3175 )