Vitamin E prevents oxidation of protein in rats hippocampal neuronal cells
Abstract：AIM:To observe effect of Vitamin E on Alzheimer's disease and study oxidative damage to mouse hippocampal neuronal cells proteins and prevent effect of Vitamin E on oxidative damage. METHODS:Using oxidative stress(H2O2) and vitamin E pretreated plus oxidative stress(H2O2) to mouse hippocampal neuronal cell line(HT-22).Ox idized proteins by 2-dimensional gel electrophoresis with silver staining and oxidation specific immunostains were measured. RESULTS:After oxidative stress 12 hours,survival rate markedly decreases to 31.19% .Cells survival rate nearly reached normal level after Vitamin E treatment followed by oxidation treatment.The number of oxidative proteins in H2O2-treated hippocampal neurons increased,while no increase was found in Vitamin E treated neurons. CONCLUSION:Vitamin E can prevent oxidation of neuronal cells in mouse.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 3046-3047 )