Influence of benzylenzyltetrahydropalmatine on the delayed rectifier potassium current by protein kinase C pathway in guinea pig ventricular myocytes
Abstract：AIM:To investigate influence of BTHP on the delayed rectifier potassium current by protein kinase C pathway in Guinea pig ventricular myocytes and its prospect in clinic. METHODS:Ik was recorded using whole cell arrangement of the patch-clamp procedure. RESULTS:Phorbol 12-myristate 13-acetate( PMA,10.0 μ mol/L) caused an enhance effect on Ik intercellularly, with increasing of Ik and Ik,tail from (13.1± 1.4) pA/pF and (5.1 ± 0.7 ) Pa/pF to (19.5± 0.7) pA/pF and (7.3± 0.4) pA/pF.Inhibition effect of BTHP on Ik was markedly augmented after pre-application of PMA.Inhibition rates of Ik and Ik,tail were increased from (38± 6)% and (36± 6)% only with BTHP to (64± 7)% and (64± 6)% in the exposure to BTHP together with PMA. CONCLUSION:Block of BTHP on Ik is suggested partly connecting with regulation of PKC in the guinea pig ventricular myocytes.BTHP extracted from medicinal plant origin is a new potassium channel blocker.Its potential as a frequency-dependent class Ⅲ antiarrhythmic agent is arousing great interest.
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Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2918-2919 )