Expression of Numb gene in cultured neural stem cells
Abstract：Aim To determine the expression and distribution of morphogen molecule-Numb participating in notch signal conduction in asymmetric cell division in cultured neural stem cells in vitro and provide new clues for differentiation controlling of neural stem cells in vitro.Methods Pregancy 14 d(E14) Balb/c inbred mice were used.Cultured neural stem cell clones which were isolated and expanded in serum-free medium in vitro were subjected to RT-PCR after being transferred several times in vitro and identified by immunochemistry.The amplification products was sequenced and labeled by digoxingemin and in situ hybridization technique was used to detect the cryosections of the neural stem cell clone.Results Large amount of cells(>95% ) in the stem cell clone could be detected positive for signal of Numb gene, there was no significant difference between the positive cells and the center and peripheral of the stem cell clone.Conclusion Morphogen molecule-Numb may play an important role in the proliferation and differentiation of neural stem cell.
Keywords：stem cellsimmunohistochemistryculture mediaserum-freenervous system/cytologynervous system/embryologycell culture
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2678-2679 )