Effect of basic fibroblast growth factor on hippocampus neurons in rat model with unilateral fimbria-fornix transection
Abstract：Aim To study the effect of basic fibroblast growth factor(bFGF) on neurogenesis in dentate gyrus of rat model of AD and to explore the application prospect of bFGF in treatment of AD with unilateral fimbria-fornix transection.Methods 36 SD rats were randomly divided into normal control,AD control and AD treatment groups with 12 rats in each group.We established rat model of AD via left fimbria-fornix transection.Normal saline was injected in normal control group.Rats in AD treatment group bFGF infusion into the cerebroventricular space every day after being lesioned for 7 days.Normal saline was injected in AD control group and normal control group.BrdU was used to label proliferated cells.DNA segments were labeled by TUNEL method and apoptic cells were detected in situ.Numbers of positive and apoptic cells at dentate gyrus were counted.Results Compared with AD control group,number of BrdU positive cells increased in AD treatment group[granular cell layer, ( 163± 37) vs ( 53± 5) /section plane;hippocampus hilum, ( 28.5± 5.5) vs ( 12.3± 2.8) /section plane;molecular layer, ( 10.7± 2.2) vs ( 6.0± 1.4) /section plane,significant differences existed( F=103.4, 83.4, 33.4, P< 0.01) while no significant difference was found in number of apoptic cells( P >0.05) .There were no significant differences in number of BrdU positive cells and apoptic cells at dentate gyrus compared between AD control group and normal control group.Conclusion bFGF can promote neurogenesis in dentate gyrus of rat model of AD.
Keywords：Alzheimer diseasefibroblast growth factorbasichippocampus
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2666-2667 )