Effect of hyperbaric oxygen on apoptosis of neuron in the CA1 area of hippocampus following forebrain ischemia-reperfusion
Abstract：Aim To study the mechanism of hyperbaric oxygen ( HBO ) on the cerebral ischemia and reperfusion injury and to provide theoretical basis for hyperbaric oxygen treatment ( HBOT ).Methods 20 gerbils weighed 60-90 g were divided by random number table into normal control,ischemia,0.15 Mpa HBO treatment,0.25 Mpa HBO treatment,0.25 Mpa hyperbaric air(HBA) control groups(n=4 in each group).The effect of 0.15 Mpa and 0.25 Mpa HBO treatment ( 60 min/d, for 3 successive days) on ischemia reperfusion-induced apoptosis in gerbil hippocampus CA1 were examined by using TUNEL staining method.Results Large amount of apoptotic neurons were found in CA1 area of hippocampus of gerbils after 3 days of ischemia and reperfusion [(163± 15)/mm2].The number of apoptotic neurons in 0.25 Mpa HBO treatment group [(73± 11)/mm2] was obviously decreased than that in 0.15 Mpa HBO treatment group [(99± 12)/mm2, P< 0.01].The number of apoptotic neurons was (151± 13)/mm2.Conclusion HBOT is effective in reducing neuronal apoptosis and in protection on neurons from injury of ischemia reperfusion, and 0.25 Mpa is an appropriate pressure for HBOT.
Keywords：hyperbaric oxygenationhippocampus/pathologybrain ischemia/pathologyneurons/pathologyapoptosisreperfusion injury/therapy
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2688-2689 )