The expression changes of glial cell line- derived neurotrophic factor mRNA after sciatic nerve cut in rats
Abstract：Aim To explore the expression changes of glial cell line- derived neurotrophic factor(GDNF) mRNA in bilateral broken ends after sciatic nerve cut in rats and its significance.Methods The sciatic nerve total RNA was extracted with Trizol one- step method and little of that was given ultraviolet scan analysis and agarose electrophoresis analysis. Others were kept with the temperature of - 80 ℃ .The specimen of sciatic nerve of approximate and distant sides was taken respectively before and after sciatic nerve severed.The level of GDNF mRNA on both cutting sides was observed and compared before and after sciatic nerve cut,using semi- quantitative RT- PCR method with β- actin as a inner consult.Results Much minim expression of GDNF mRNA was found on sciatic nerve before operation ( 6.5± 1.3)% , but it increased ( 7.8± 1.9)% of the expression quantity 24 hours after sciatic nerve cut,( 10.3± 2.1)% 7 d,( 11.9± 2.3)% 14 d and ( 12.3± 2.4)% 28 d,while it decreased ( 5.8± 1.3)% ,( 4.0± 1.0)% , ( 3.6± 1.1)% and ( 3.1± 1.0)% on 1,7,14 and 28 d respectively.1 d after sciatic nerve cut compared with before operation( P >0.05,t=1.193,0.879) ,7,14,28 d after sciatic nerve cut compared with before operation(P< 0.01,t=3.762,3.565,5.059,4.083,5.164,4.905). Conclusion The expression of GDNF increases sharply under the stimulation of injury signals then the neurons can be recovered,which provides theoretical foundations for GDNF to be used in treating spinal cord injury.
Keywords：brain- derived neurotrophic factorsciatic nerve/ injuriesspinal cord injuriesmotor neuronsgene expression
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2394-2395 )