Significance of dexamethasone- induced apoptosis of thymocytes in immuno- neurological diseases
Abstract：Aim To investigate the expression of p38MAPK and caspase- 3 in dexamethasone- induced apoptosis of thymocytes and explore the significance of dexamethasone- induced apoptosis of thymocytes in immuno- neurological diseases.Methods The mouse thymocyte model of apoptosis was induced by dexamethasone. The apoptosis ratio was observed with flow cytometry technique. The changes of caspase- 3 protein and the expression of p38MAPK proteins were detected by western blotting at 0,30,60 and 180 min after dexamethasone- induced thymocytes apoptosis.Results The apoptosis ratio was 21.75% .There was expression of caspase- 3 at 0 min after dexamethasone- inducing,then was gradually increased following time lapse,kept high till 180 min.The expression of p38MAPK was detected immediately, and reached peak at 60 min, then decreased at 180 min after dexamethasone treatment.Conclusion p38MAPK signal passage plays an important role in dexamethasone- induced thymocytes apoptosis.
Keywords：dexamethasonecystein endopeptidasesprotein kinases
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2312-2313 )