Relationship between fastigial nucleus electrical stimulation and calpain activity during focal cerebral ischemia and reperfusion
Abstract：Aim To investigate the influence of fastigial nucleus electrical stimulation on the activity of calpain during focal cerebral ischemia and reperfusion and identify the neural protective mechanism of fastigial nucleus electrical stimulation.Methods 120 healthy male Wistar rats weighed 250- 300 g were randomly divided into pseudo- operation group(PO),FN electrical stimulation pseudo- operation group(PO- FN),ischemia/reperfusion group(I/R) and ischemia/reperfusion- FN stimulation group(I/R- FN).The later two groups were divided into 1,3,6,12,24 h groups respectively with 10 rats in each groups.FN electrical stimulation and MCAO and reperfusion model of rats were established.Using semi- quantitative Western blotting for detecting the content of 150 RU FBDP (Calpain- mediated fordrin breakdown pruduct)and Nissl's staining for scoring by neuronal number and size,we observed the changes of calpain activity and neuronal injury in reperfusion area after different time of focal cerebral ischemia and reperfusion in rats with or without FN electrical stimulation.Results Calpain activity increased obviously in I/R group compared with those in I/R- FN group [(6 310± 360) ,( 4 660± 310) /μ g total protein and ( 7 060± 290) ,( 6 240± 310) /μ g total protein, t=7.776,P=0.000 and t=4.267,P=0.003] .After 6- 24 h of reperfusion,calpain activity in I/R group increased gradually which had a statistical significance compared with that in I/R- FN group(P=0.000 in each group).In I/R group,score of neuron number and size increased dramatically from 1 h of reperfusion(2.1± 0.2), increased gradually and reach climax at 24 h(4.0± 0). Score of neuron number and size in I/R- FN group increased lightly at 1- 3 h of reperfusion,then became stable.Shrinkage of neurons became more obvious at 24 h of reperfusion,score of neuron number and size increased again,but scores in each group were significantly lower than those in I/R group at each phase (z=2.356,P=0.018; z=2.008,P=0.045; z=2.887,P=0.004; z=2.685,P=0.007; z=2.835,P=0.005).Conclusion FN electrical stimulation can inhibit calpain activity during focal cerebral ischemia and reperfusion and protect the neuron from reperfusion injury.
Keywords：cerebellar nucleiprotein kinasesreperfusionbrain ischemia
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2276-2277 )