Expression of growth- associated protein(GAP- 43)and its mRNA in injured rat sciatic nerve
Abstract：Aim To study the expression of growth- associated protein (GAP- 43) and its mRNA in distal stump of rat injured sciatic nerve and to provide experimental basis for improving microenviorment.Methods Western Blot and RT- PCR were used to detect the different expression of GAP- 43 and its mRNA in the distal stump of injured sciatic nerve.Results RT- PCR result,the expression of GAP- 43 mRNA was in low levels in the normal sciatic nerve and increased significantly after nerve injury,and reached climax at the second week after the sciatic nerve was injured.Western Blot results with anti- GAP- 43 antibody,the special positive band of 43 kDa protein was light in normal sciatic nerve and deep staining positive band of 43 kDa protein in the distal stump of injured sciatic nerve, and reached the peak at the third week.Conclusion The expression of GAP- 43 and its mRNA are increased significantly in distal stump of injured sciatic nerve.The up- regulation peak of GAP- 43 mRNA is at the second week,the peak of the protein synthesis is at the third week, and its up- regulation is impressed by nerve injury.
Keywords：GAP- 43 proteinsciatic nervewounds and injuriesrats
Publication Date：2003-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2304-2305 )